ANTIOXIDATIVE STATUS AND LIPID PEROXIDATION IN RATS AFTER ADMINISTRATION OF UKRAIN
MELNICHENKO N., SUDNIKOVICH E., MAKARCHIKOV A., ZVERINSKY I.
Institute of Biochemistry, Grodno, Belarus.
Address for correspondence: Dr. I. Zverinsky, Institute of Biochemistry, 230017, BLK-50, Grodno, Belarus.
Summary: The effect of Ukrain on the antioxidant function of rat liver and on lipid peroxidation intensity was studied. Thirty male Wistar rats weighing 180-200 g were used. The animals were divided into three groups of 10. The first (control) group received injections of normal saline solution in doses of 2 ml/kg body weight; the second and third groups were injected with Ukrain at doses of 1 mg/kg and 2 mg/kg, respectively. All injections were intraperitoneal, daily, for 6 days. Reduced glutathione content, antioxidant activity, defining the ability to prevent peroxidation reactions, the catalytic activity of glutathione-peroxidases on cumyl and tret-butyl substrates and the generation rates of initial and end lipid peroxidation metabolites (lipid hydroperoxides and mal-ondialdehyde, respectively) were estimated. On day 7 after Ukrain administration at a dose of 2 mg/kg, the serum concentration of reduced glutathione and serum antioxidant activity had significantly decreased by 25% and 49%, respectively. At the same time, in the postmitochondrial fraction of rat liver, GSH reductase activity increased by 43% compared with the control, probably as a metabolic adaptive response to the GSH decrease.