THE IMMUNOMODULATING PREPARATION UKRAIN DOES NOT INDUCE ANAPHYLACTIC SENSITIZATION IN MICE AND GUINEA PIGS

WYCZÓŁKOWSKA J.,* CZUWAJ M., MAŚLIŃSKI C.

Department of Biogenic Amines, Polish Academy of Sciences, Tylna 3, 90-950 Łódź, Poland.

* Author to whom correspondence should be addressed.

Summary: The ability of Chelidonium majus L. alkaloids derivative Ukrain to induce an anaphylactic sensitization was tested on mice and guinea pigs. The levels of IgE antibody in the mouse sera, and IgG_1a, IgG_1b as well as IgE antibody levels in guinea pig sera, were evaluated by passive cutaneous anaphylaxis (PCA) tests. Ukrain alone or adsorbed on aluminium hydroxide gel (alum) introduced into BALB/c mice in several subcutaneous injections was unable to stimulate measurable anti-Ukrain IgE antibody response. Moreover, Ukrain introduced together with ovalbumin (OA) into mice in the course of immunization with OA induced lower anti-OA antibody response as compared to the response induced by OA alone. Ukrain adsorbed on alum and injected subcutaneously into guinea pigs did not induce measurable IgG_1a, IgG_1b and IgE antibody response. The present results suggest that the immunomodulating preparation Ukrain could be therapeutically safe at least as far as its inability to induce anaphylaxis is concerned.