PRELIMINARY OF PHARMACOKINETIC STUDIES OF UKRAIN IN RATS

JAGIEŁŁO-WÓJTOWICZ E.,¹* KLEINROK Z.,¹ CHODKOWSKA A.,¹ MISZTAL G.,² JAGIEŁŁO G.³

1) Department of Pharmacology, Medical Academy , Jaczewskiego 8, 20-090 Lublin, Poland.
2) Department of Medicine of Chemistry, Medical Academy, Chodźki 6, 20-090 Lublin, Poland.
3) Institute of Applied Mathematics, University of Agriculturae, Akademicka 13, 20-934 Lublin, Poland.

*Author to whom correspondence should be addressed.

Summary: Ukrain (thiophosphoric acid alkaloid derivatives from Chelidonium majus L.) was intraperitoneally (ip) administered in a dose of 28 mg/kg (equivalent to 0.1 LD₅₀) into the rat. A HPLC method for a rapid determination of Ukrain in plasma was described.

It was found that Ukrain rapidly penetrated into the plasma of rat and the elimination of drug from the plasma was slower. The results obtained were as follows: 1/ absorption rate constant: k_a = 0.0432 [min^-1], 2/ elimination rate constant: k = 0.0113 [min^-1], 3/ time of the drug half-life: t_1/2 = 61.32 min, 4/ the actual Ukrain concentration in the plasma: C = 33 e^-0.0113t - 39 e^-0.0432t [μg/ml] 5/ time of delaty of the drug absorption: T₀ = 5.23 min .